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Effect of Low-Level Laser Radiation on Some Rheological Factors in Human Blood: An in Vitro Study

Dan G. Siposan, Adalbert Lukacs. Journal of Clinical Laser Medicine & Surgery. August 1, 2000, 18(4): 185-195. doi:10.1089/10445470050144038. Dan G. Siposan Doctoral candidate Technical Military Academy, Bucharest, Romania

Adalbert Lukacs Doctoral candidate Center of Transfusion Hematology of the Army, Bucharest, Romania

Objective: The purpose of this study was to investigate the in vitro effects of low-level laser radiation (LLLR) on some rheological factors of the human blood, such as complete blood count (CBC) parameters and blood sedimentation rate (BSR). We were mainly concerned with the alterations caused by LLLR action on blood cells (erythrocytes and leukocytes) of fresh blood obtained from apparently healthy adult patients. We used low doses ranging between 0.80 Jââ?¬Â¢cm-3 and 4.40 Jââ?¬Â¢cm-3, at the very low-power densities of the laser radiation, so as not to damage the cell structure and not to alter in an undesired manner their functions.

Methods: Blood samples were taken from 22 volunteers. Where health problems existed, they were indicated for each case. The parameters mentioned above were measured before (control samples) and after irradiation. A He-Ne laser, operating in a continuous wave, as a radiation source (632.8 nm, 1 mW, mean power density incident on blood samples around 30 mW cm-2, beam spot diameter 2 mm) was used. The measurements were performed immediately after irradiation. Only the erythrocyte complex was irradiated. EDTA anticoagulant was used. Results: The measurements using a computerized hemoanalyzer type SERONO showed significant differences between control and irradiated blood samples concerning the following parameters: RBC (in 22% of cases), HGB (47.3%), HCT (84.2%), RDW (11%), PLT (5.26%), MPV (33.3%), WBC (5.26%), MONO (26.3%), and GRAN (63.15%). In the case of BSR (44%), the significant differences were noticed especially in the cases of patients suffering from some acute or chronic diseases. No significant differences were noticed in the cases of MCV, MCH, MCHC, RDW, and LYMPH.

Conclusions: This study has shown that LLLR, even though used at low doses and low power densities, produced some changes of the rheological factors of the blood, as follows: a revitalizing and regenerating effect on mitosis stimulation and a nondamaging and biostimulating effect on the cell membrane (by keeping unmodified MCV, MCH, and MCHC). In 3 cases out of 22, hemolysis (complete or partially) occurred, but we are not yet sure whether this was caused by laser exposure or by certain environmental physical factors

Blood Trauma Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat blood trauma. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions.

Intravascular laser irradiation of blood in treatment of traumatic abdominal organs injuries

Koshelev VN, Chalyk IuV.

Khirurgiia (Mosk). 1998;(5):40-2.

The application of intravascular laser irradiation of the blood (ILIB) in combined postoperative intensive care in 28 patients with traumatic injuries of parenchymal organs and severe intraabdominal bleeding (more than 20% CBV) contributed to substantial improvement of the results of treatment and reduction of complications rate. ILIB boosts functional activity of leucocytes, normalizes the system of hemostasis and antioxidant defense.

Laser radiation to correct disorders of blood albumin transport in severe mechanical trauma

Kravchenko-Berezhnaia NR, Moroz VV, Kozhura VL.

The paper provides evidence for that it is expedient to perform multi-stage intravascular low-intensity laser blood radiation in patients with severe mechanical trauma and massive blood loss in the early posttraumatic period. The use of laser radiation at a wavelength of 632.0 nm by inserting a disposable light guide (its end power was 1.5-2.0 mW, and the duration of a session–30 min) as part of complex therapy in this group of patients romotes the increase of plasma albumin transport ability and the general stimulation of natural detoxifixation mechanisms.

Acute Respiratory Distress Disorder Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Acute Respiratory Distress Disorder. These studies are presented here to demonstrate the wide uses of cold lasers in the treatment of different medical conditions. Acute Respiratory Distress Syndrome Successfully Treated with Low-Level Laser Therapy Chan Gunn, Institute for the Study and Treatment of Pain Abstract Acute respiratory distress syndrome (ARDS) is a medical emergency, which may be

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Alcoholism Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Alcoholism. These studies are presented here to demonstrate the wide uses of cold lasers in the treatment of different medical conditions. Acupuncture for alcohol withdrawal: a randomized controlled trial 1 Psychiatric Hospital,2 Department of Complementary Medicine (KIKOM), Inselspital, University of Berne, 3010 Berne, 3 Department of Social and Community Psychiatry, University of Berne, Laupenstrasse 49, 3010 Berne, Switzerland,

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Alzheimer’s Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using a cold laser to treat Alzheimer’s Syndrome. These studies are presented here to demonstrate the wide uses of a cold laser in the treatment of different medical conditions. The Efficacy of 904 nm Laser Therapy for Alzheimer’s Diseases Kazuyoshi Zenba, Vice president of Kanagawa Acupuncture Massage Association Prof. Masayuki Inoue, Secretary of JLPLTPA Preface: Although we had reported about the possible

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Application of laser acupuncture in the treatment of periarthritis humeroscapularis

Branka Nikolic,. spec. Sport’s med. Address Zavod za zdravstvenu zastitu radnika ZTP-a “Beograd“, 11000 Beograd, Slobodana Penezica Dr. 23 Yugoslavia The effect of low intensity semi conductor laser was used as treatment methods for periarthritis humeroscapularis. The CC laser (Computer Controlled laser) was applied. Laser therapy has positive biological effects and antiinflamatory, antioedema effects and analgesia. We treated 18 patients with periarthritis humeroscapularis, 14 were female patients. The laser was locally applied at the AC points Sj 14, Sj 15, Li 15, Li 10, Sj 5, Si

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Arteriosclerosis Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Arteriosclerosis. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. Low Level Laser Therapy in the Treatment of Arteriosclerosis of the Lower Limbs Attia M.A., EI-Kashef H. Laser center, Alhikmah Hospital, EI-Mansoura, Egypt Physics Department, Faculty of Science, Tanta Egypt Abstract: Twenty patients with arteriosclerosis

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Arthritis-Cervical Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Arthritis-Cervical. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. The clinical efficacy of low-power laser therapy (lllt) on pain and function in cervical osteoarthritis. Ozdemir F, Birtane M, Kokino S. Department of Physical Therapy and Rehabilitation, Medical Faculty of Trakya University, Edirne, Turkey.Clin

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Arthritis-Rheumatoid Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using a cold laser to treat Arthritis-Rheumatoid. These studies are presented here to demonstrate the wide uses of cold lasers in the treatment of different medical conditions. 50 year old female patient diagnosed as having Class 3 RA inflammatory phase of steady development. Probes used in treatments were 820nm (50mW) and 31 cluster probe with the 820nm probe being administered on each interphalangeal joint (30 seconds per

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Asthma Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Asthma. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. Treatment of bronchial asthma with low-level laser in attack-free period at children Ailioaie, C.; Ailioaie, Laura AA(Univ. of Medicine and Pharmacy)Proc. SPIE Vol. 4166, p. 303-308, Laser Florence ’99: A Window on the Laser

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Bronchitis Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat bronchitis. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. The use of the low-level laser therapy (LLLT) in the treatment of some pulmonary diseases (10 – years experience) DERBENJEV V. A. (1) ; MIKHAILOV V. A. (1) ; DENISOV I. N. (2) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s) (1)

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Burn Scars Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat burn scars. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. Low Level Laser Therapy’s conservative approach to the burn scar K . Gaida / Burns , Volume 30 , Issue 4 , Pages 362 – 367 Burn scars are known to be difficult to

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Candida Albicans Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat candida albicans. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. Effect of low-level laser therapy on Candida albicans growth in patients with denture stomatitis. Maver-Biscanin M, Mravak-Stipetic M, Jerolimov V. Department of Prosthodontics, Clinical Hospital Centre, Zagreb, Croatia. mirela.maver@zg.htnet.hrPMID: 15954824 [PubMed – indexed for MEDLINE

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Clinical application of GaAIAs 830 NM diode laser in treatment of rheumatoid arthritis

Kanji Asada, Yasutaka Yutani, Akira Sakawa and Akira Shimazu. Department of Orthopedic Surgery, Osaka City University Medical School, Japan The authors have been involved in the treatment of rheumatoid arthritis (RA), in particular chronic poly-arthritis and the associated pain complaints. The biggest problem facing such patients is joint contracture, leading to bony ankylosis. This in turn severely restricts the range of motion (ROM) of the RA-affected joints, thereby seriously restricting the patient’s quality of life (QOL). The authors have determined that

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Effect of Low-Level Laser Radiation on Some Rheological Factors in Human Blood: An in Vitro Study

Dan G. Siposan, Adalbert Lukacs. Journal of Clinical Laser Medicine & Surgery. August 1, 2000, 18(4): 185-195. doi:10.1089/10445470050144038. Dan G. Siposan Doctoral candidate Technical Military Academy, Bucharest, Romania Adalbert Lukacs Doctoral candidate Center of Transfusion Hematology of the Army, Bucharest, Romania Objective: The purpose of this study was to investigate the in vitro effects of low-level laser radiation (LLLR) on some rheological factors of the human blood, such as complete blood count (CBC) parameters and blood sedimentation rate (BSR). We were

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Laser Therapy and Musculoskeletal Pain Syndromes (Chronic and Acute)

Musculoskeletal Pain Syndromes (Chronic and Acute) Low-Level Light Therapy (LLLT) has been shown to be effective in a variety of musculoskeletal conditions and associated pain presentations. In Rheumatoid Arthritis, LLLT can benefit not only the pain of acute small joint inflammation but also chronic pain. In a review article on rheumatology (3), some 18 papers were considered. All studies involved double-blind trials with LLLT in chronic rheumatoid and reported significant improvement in pain (80% success

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Laser Therapy Science & Clinical Studies

Cold Laser Therapy Science, Clinical Studies,Education Cold Laser Therapy has been practiced for over 50 years. There are over 5000 Proof Positive Clinical Studies and Research Papers that reveal beyond a shadow of a doubt that laser therapy, phototherapy aka Photobiomodulation (PBM) is effective at treating a wide range of conditions that were previously untreatable, except sometimes through drugs and surgery. There are over a quarter million health care providers using cold lasers in their

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LLLT using a diode laser in successful treatment of a herniated lumbar/sacral disc, with magnetic resonance imaging (MRI) assessment

Tatsuhide Abe. Abe Orthopaedic Clinic Futuoka City Fukuoka Prefecture Japan X12′ A 40- year-old woman presented at the Abe Orthopedic Clinic with a 2-year history of lower hack pain and pain in the left hip and leg diagnosed as a ruptured disc between the 5th lumbar/lst sacral vertebrae. The condition had failed to respond to conventional treatment methods including pelvic traction, nonsteroid anti-inflammatory drugs and aural block anesthetic injections. MRI scans were made of the affected disc, showing it protruding on the left side through the aural membrane. The

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Low level laser therapy of tendinitis and myofacial pains a randomized, double-blind, controlled study

Mimmi Logdberg-Anderssont (1), Sture Mutzell (2), and Ake Hazel (3) 1: Akersberga Health Care Centre,2: Danderyd University Hospital, Danderyd, and3: Vaxholm Health Care Centre, Stockholm, Sweden. The purpose of this randomised, double-blind study was to examine the effect of GaAs laser therapy for tendonitis and myofascial pain in a sample from the general population of Akersberga in the northern part of Greater Stockholm. 176 patients (of an original group of 200) completed the scheduled course

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Mechanisms of the analgesic effect of therapeutic lasers in vivo

L Navratil (1) and I Dylevsky (2) 1: Outpatient Department of Radiobiology, Institute of Biophysics, First Medical Faculty, and 2: Department of Functional Anatomy, Second Medical Faculty and Faculty of Physical Education Charles University, Prague, Czech Republic The analgesic effects in the course of application of therapeutic lasers to affected tissue have been described in a number of works in the literature. Although a few scientific- based reports have appeared, those on laser-induced analgesia are mainly clinical works describing the

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Mechanistic approach to GaAIAs diode laser effects on production of reactive oxygen species from human neutrophils as a model for therapeutic modality at cellular level

Makoto Yamaya*, Chiyuki Shiroto’, Hiroki Kobayashi*, Shinji Naganuma*, Jyuichi Sakamoto*, Koh-Jun Suzuki*, Shigeyuki Nakaji*, Kazuo Sugawara* and Takashi Kumae *Department of’ Hygiene, Hirosaki University School of Medicine. Hirosaki; .-Shiroto Clinic Coshogawara, Aomori: Department of Industrial Health. The Institute of Public Health, Tokyo. Japan. There have been many reports on the applications of low reactive level laser (LLL) therapy for pain attenuation or pain removal. Our group has reported previously on the effects of in vitro irradiation of LLLT particularly on the phagocytic activity of human Neutrophils, using luminol-dependent chemiluminescence (LmCL) for measurement of reactive oxygen species

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Pain scores and side effects in response to low-level laser therapy (LLLT)/ Cold Laser Therapy for physical trigger points

E Liisa Laakso Carolyn Richardson, and Tess Cramond 1: Physiotherapy Department, Royal Brisbane Hospital, Brisbane; 2: Physiotherapy Department, University of Queensland, Brisbane; and 3: Pain Clinic, Royal Brisbane Hospital, Brisbane, Queensland, Australia. Clinically, Low Level Laser Therapy – LLLT has been used successfully in the treatment of chronic pain but many have questioned the scientific basis for its use. Many studies have been poorly designed or poorly controlled. A double-blind, placebo-controlled, random allocation study was

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Physiological responses in chronic pain patients LLLT protocol

Scott D. Fender and David Diffee. Pain Research Group, Arvada, Colorado, U.S.A. Use of Low Reactive Level Laser Therapy (LLLT) utilizing helium-neon lasers has increased lately especially in pain control. New protocols are being developed aimed at a complex of primary and secondary symptomologies. One of these protocols, Stellate Ganglion Stimulation, has shown in our research a unique set of developments. Targeting the area of the stellate ganglion is showing great promise in the rehabilitation of

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The Effects of Laser Therapy in the Early Stages of Rheumatoid Arthritis Onset

C. Ailioaie, M. D. Medical Office for Laser Therapy, Iassy, ROLaura Marinela Lupusoru-Ailioaie, M. D.“Al.I.Cuza” University, Dept. of Medical Physics, Iassy, RO 1.PURPOSE: To study the effects of laser therapy, in comparison with other modality trials (NSAIDs), at the onset of (RA). 2.SUBJECTS and METHODS: In the study 59 patients were included, in the first 6 – 12 months from RA onset. The patients were divided into three groups: Group 1 (21 patients) received laser therapy; Group 2 (18 patients)

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